Additionally, we compared the responses of both systems (ddPCR
vs. qPCR) to the presence of inhibitory substances, such as sodium
dodecyl sulfate (SDS), ethanol and ethylenediaminetetraacetic acid
(EDTA). Lastly, ddPCR was employed in the generation of GMstandards
with varying concentrations of the transgene event,
namely in a 0.1e10% range (specifically 0.1%, 0.5%, 1% and 10%),
employing 100% GM free and 100% GM plant material.