Automatic genome annotation using the RAST server (3), based on the CLC Genomics Workbench-derived contigs, gave a 39% subsystem coverage for 5,251 putative coding sequences. An analysis of the average nucleotide identity (9) across complete genome sequences of P. polymyxa strains (E681 and SC2) and draft sequences of P. polymyxa ATCC 842T and P. peoriae KCTC 3763T revealed that P. peoriae KCTC 3763T is closer to E681. In the draft sequences, we were able to identify gene clusters for the biosynthesis of tridecaptin and a novel heptapeptide antibiotic that is similar to hexapeptide fusaricidin antibiotics, along with several contigs harboring NRPS genes encoding unknown products. We were also able to determine several genes for extracellular carbohydrases, including an amylase, a xylanase, a cellulase, and several glucanases, which may be responsible for the utilization of plant-derived polysaccharides in the rhizosphere.