The phenolic acids in phenolic fractions after acid hydrolysis of
raw and ripe mango peels were separated on reverse phase C18
column on HPLC using water: methanol: acetic acid (83:15:2).
The compounds eluted were monitored at 280 and 320 nm to detect
various compounds. The retention times of these peaks were
compared with the authentic standards of phenolic acids. However,
wherever there is a little variation in retention times, their
identifications were confirmed by spiking with authentic standards.
Gallic acid, protocatechuic acid, gentisic acid and syringic
acid were the phenolic acids identified in phenolic fractions of
raw and ripe mango peel extracts.