The purpose of this study was to find discrimination markers for four major meat species such as beef,
pork, chicken and duck. Myofibrillar and sarcoplasmic proteins isolated from each meat type were
analyzed by one-dimensional gel electrophoresis and some proteins were identified through LC-MS/MS
analysis. We confirmed that troponin I (TnI), enolase 3, L-lactate dehydrogenase (LDH) and
triose-phosphate isomerase (TPI) could be useful markers for discrimination of mammals from poultry
due to their different electrophoretic mobility. Tropomyosin 1 and carbonic anhydrase 3 were observed
as muscle fiber type-related proteins and these could also be markers to distinguish mammals from
poultry. Species-specific peptides identified by LC-MS/MS spectra allow the identification of each species
regardless of the same protein. Therefore, it is easy to discriminate between mammals and poultry by
comparing the electrophoretic mobility of TnI, enolase 3, LDH, TPI and CA3, and each species could be
identified through LC-MS/MS analysis.