A multi-layer structure with micro-topological morphology was constructed and further surface chemical modification was made for flame sprayed aluminum coatings. The C. closterium diatoms tend to settle on the areas around the locations with protruded topographical features. The zwitterionic polymer-modified sur- face brought about marked retardation in protein adsorption and diatom adhesion. The micropatterned surfaces show enhanced antimicrobial performances, while the additional pSB surface treat- ment plays the most significant role in promoting the capability of the coatings to resist settlement of the diatoms on their surfaces. This study provides a simple yet efficient platform for investi- gating the combined effects of surface topography and chemistry on the colonization behaviors of marine diatoms on marine infrastructures.