In a 1 l reactor, equipped with a mechanical agitation and
a jacket, 500 ml of an aqueous ethanol solution was introduced.
At initial time, a known mass of A. paniculata was
introduced in the reactor and the extraction was performed
at 22 ◦C during 3 h. Solid and liquid phase were then basically
separated with a filter just to obtained most of the liquid
phase. The solution was then heated to its boiling point
and samples were taken and analyzed in order to obtain the
kinetic of destruction of andrographolide. Three initial solutions
were studied: 100% of water, 75 and 15% of ethanol
so the destruction were done at three different temperatures:
97, 92 and 86 ◦C, measured in the reactor.