The loss in weight on incineration = W1– W2.
%Crude fiber = W1 – W2 x 100
Weight of original sample
Determination of Total Lipid Concentration Using
Solvent Extraction (Using Soxhlet Method).
The soxhlet extraction method used is as described in
AOAC
[9]).
A fat free thimble was weighed W1. A clean
250cm3
round bottom flask with some boiling chips was
also weighed WA. 6g of the sample was weighed into the
thimble, W2. This was placed in a soxhlet extractor; 200mls
of petroleum ether was poured into the flask. The
condenser was then connected to the extractor and the
flask which was a heating mantle. The heat was then
increased slowly until the solvent began to boil. The
extraction was left to ―run‖ through ten refluxes. (A reflux is
when enough solvent has been collected in the soxhlet
cylinder to be siphoned back into the round bottom flask).
The thimble and its content were dried overnight in the oven