and then snap chilled in crushed ice. The obtained lysate (5μl) was used as a DNA template in PCR reaction mixture. Bacterial DNA was also extracted employing DNA extraction kit (Mini Prep, Sigma).
and then snap chilled in crushed ice. The obtained lysate(5μl) was used as a DNA template in PCR reaction mixture.Bacterial DNA was also extracted employing DNAextraction kit (Mini Prep, Sigma).