In the enzyme load study, glycerolysis of sardine oil was carried out using Lipozyme® 435 at a load of 5 and 10 wt% based on reactants weight, with a glycerol/oil mole ratio of 3:1. The results are shown in Fig. 1B. As expected, the MAG yield increased with increasing lipase concentration, which may be due to higher number of active sites. MAG production was around 90% and 80% (expressed free of glycerol and TP) at 10% and 5% of lipase loading, respectively