In order to evaluate the precision and accuracy of the analytical method for the determination of the cholesterol content in oils and fats, a repeatability and recovery test (n = 5) was carried out. According to the relevant literature and our previous study, the cholesterol concentration in the VCO samples was less than 5 mg/kg, and the level of cholesterol in animal fats, such as LD, CF, BT, or MT, ranged from 300 mg/kg to 2000 mg/kg