Chromatographic conditions
Standardization of clindipine by RP-HPLC method was
carried out using the optimized chromatographic
conditions. The mobile phase used was acetonitrile:
05mM potassium dihydrogen orthophosphate (ph 4.5).
Potassium dihydrogen ortho phosphate used was 5 mM
solution in water with pH being adjusted to 4.5 with
orthophosphoric acid solution. The injection volume was
20.0 μL. The UV-visible detector was set at 260 nm.