Highlights
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Determination of proline in honey: first-time comparison between official methods.
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The AOAC method proves to be the best in terms of accuracy and of time-saving.
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Optimization of the waiting time for absorbance recording.
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Method validation: LOD 20 mg kg−1, LOQ 61 mg kg−1, linearity up to 1800 mg kg−1.
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Unifloral honey Marche region: good quality, mature and unadulterated.