2.5. Preparation of ethanolic extracts
Cookie powder (5 g) was mixed with 50 mL of ethanol/water
(80/20, v/v). Extraction was carried out by shaking the mixture at
room temperature (23 ± 1 C) for 1 h. After 1-h shaking, the suspension
was left overnight at room temperature. The procedure
was repeated twice with 50 mL of solvent, and combined extracts
were dried using a vacuum-evaporator. The dried extract was dissolved
in ethanol/water (80/20, v/v) to 10 mL volume and used for
further investigation of antioxidant activity.