640 soil samples were collected from various agroclimatic zones of India and 250 isolates of Trichoderma isolated from them were morphologically characterized. The thermotolerant isolates identified from the stress tolerance studies were characterized by sequencing a section of elongation factor 1 alpha gene. According to NCBI BLAST search against the GenBank sequence database and comparison using TrichoBLAST and TrichoKey tools (Druzhinina et al. 2005), most of the isolates were identified as T. asperellum and their corresponding accession numbers were obtained from the NCBI. The geographical location of these isolates was also listed for reference (Table 1).