biochemical estimations were carried out using automated analyser (Hitachi 902; roche, manheim, Germany) and commercial kits (roche). The normal range for serum total calcium, (8.8-10.2 mg/dl), ionic calcium. (1.12-1.32 mmol/L), inorganic phosphorus (2.7-4.5 mg/dl), and alkaline phosphatase were (females: <240 U/L; males: <270 U/L). The serum concentrations of 25(oH)D (reference range: 9.0-37.6 ng/ml) and PTH (reference range: 10-65 pg/ml) were measured by rIA (Diasorin, Stillwater, mn) and electrochemiluminescence assay (roche diagnostics, GmDm-manheim, Germany) respectively.. Serum 25(OH)D level of 20.0 - <30.0 ng/ml was classified as vitamin D insufficiency1 (vDI), and levels < 20 ng/ml were classified as vitamin D deficiency (VDD). VDD was further categorized based on Lips classification as mild (10.0 – <20.0 ng/ml), moderate (5.0-<10.0 ng/ml) and severe (< 5.0 ng/ml).2 Secondary hyperparathyroidism was defined by serum PTH level of >65pg/ml.
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