Total Plate Count (TPC), Optical Density (OD) and sugar reduction concentration analysis were conducted on pre and post incubation. TPC was determined using the facilities of microbiology laboratory at UPT BPPTK LIPI. OD determined using UV-VIS spectrophotometer at UPT BPPTK LIPI on 660 nm wavelength. Sugar reduction concentration was taken with Nelson Methods using UV-Visible spectrophotometer on 540 nm wavelength [4]. Ethanol concentration was determined using Gas Chromatography (GC) type HP 5890 at Mathematics-Chemistry Laboratory-UGM Yogyakarta.