Additionally, Principal Component Analysis – PCA of 2-D
DIGE involving only the differentially abundant proteins was
carried out and presented in Fig. 2, where the score plot is
emphasized in Fig. 2A and the loading plots in Fig. 2B. Cluster
analysis of the protein abundance was then used to identify
groups of proteins, which were higher or lower abundantly (at
1.8 regulation factor of 90% variation) in T or NT soybeans
leaves.When plotting PC1 × PC2 an effective separation in their
original groups was allowed, once these two first PCs explained
97.5%of the biological variability, PC1 being accounted by 85.2%
and PC2 by 12.3%. The effectiveness of this differentiation is
also notedwhen the loading plot graphicwas observed (Fig. 2B),
Fig. 1 – 2-D DIGE gels of protein species from transgenic (T)
soybean leaves, in red, and non-transgenic (NT) soybean
leaves, in blue.
Fig. 2