4. Conclusions
Although many methods have been developed for determination of AA or TAA, both HPLC and UPLC methods proposed in this study for determination of AA or TAA (vitamin C) in fruit beverages and pharmaceutical preparations can be recommended as rapid, precise and sensitive. Extraction of AA and DHAA from tested products using 10% meta-phosphoric acid guaranteed determination of vitamin C within 24 h without its essential loss. For fresh commercial beverages as well as for pharmaceutical preparations, it is not necessary to determine both AA and DHAA. Determination of vitamin C in stored fruit juices may require determination of TAA because oxidation of AA may occur during storage. Both methods can be useful in the routine qualitative and quantitative analysis of vitamin C in beverages and pharmaceutical preparations. However, UPLC method is faster, more sensitive, consumes less eluent and it is more eco-friendly than the conventional HPLC method. In consequence, UPLC is cheaper than HPLC because a higher number of analyses per unit of time can be performed and consumption of eluent is much lower.
4. Conclusions
Although many methods have been developed for determination of AA or TAA, both HPLC and UPLC methods proposed in this study for determination of AA or TAA (vitamin C) in fruit beverages and pharmaceutical preparations can be recommended as rapid, precise and sensitive. Extraction of AA and DHAA from tested products using 10% meta-phosphoric acid guaranteed determination of vitamin C within 24 h without its essential loss. For fresh commercial beverages as well as for pharmaceutical preparations, it is not necessary to determine both AA and DHAA. Determination of vitamin C in stored fruit juices may require determination of TAA because oxidation of AA may occur during storage. Both methods can be useful in the routine qualitative and quantitative analysis of vitamin C in beverages and pharmaceutical preparations. However, UPLC method is faster, more sensitive, consumes less eluent and it is more eco-friendly than the conventional HPLC method. In consequence, UPLC is cheaper than HPLC because a higher number of analyses per unit of time can be performed and consumption of eluent is much lower.
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