An aqueous solution (5 mL) containing 10 μL of cod oil, 0.25 mmol of trizma buffer (pH 7.4), 5 μmoL of ferrous chloride, 10 μmoL of hydrogen peroxide, 0.75 mmol of potassium chloride, and 0.2% sodium dodecyl sulfate was incubated with various amounts of the volatile extracts (prepared by sequentially diluting the standard extract with ethanol) and their components
(authentic chemicals) for 18 h at 37 °C in a 20 mL test tube