L-Asparaginase producing endophytes were detected from
the plate assay by the formation of pink zone around the colony.
The reaction of L-asparaginase that hydrolyzed L-asparagine
into ammonia increased the pH of the media which was in
turn detected by phenol red, a pH indicator. Isolates incapable
of expressing L-asparaginase enzymes have agar media which
remained yellow in color. The correlation coefficient (r) used
to explain the relationship between pink zone diameter and
asparaginase activity