Fig. 4A shows the reducing sugar released after enzymatic hydrolysis by Termamyl 120L different concentrations.
The results indicated that the higher the enzyme concentration was, the higher the fermentable reducing sugar content.
The use of Termamyl 120L(45 U) released 14.16 g L−1 reducing sugars after a 60 min of incubation.
Izmirlioglu and Demirci (2012) indicated that the liquefaction stage alone was insufficient and a supplementary saccharification stage was required.
For this reason, the use of enzyme combination (Termamyl 120L and amyloglucosidase) was necessary for an effective PPW hydrolysis.
Therefore, reducing sugars release was determined using 45 U of Termamyl 120L combined to different concentrations of amyloglucosidase concentrations (Fig. 4B).
The enzyme combination with the highest release of reducing sugars (51 g L−1) was Termamyl 120L 45 U + amyloglucosidase 9 U.
At higher enzymes doses, no significant increase in reducing sugars release was obtained.
Therefore, this combination was retained to optimize the best PPW concentration for optimal release of reducing sugars.
Different concentrations (ranging from 1.25% to 17.5%) were tested (Fig. 4C).
The results showed that at low PPW concentration, very low amounts of reducing sugars were released but increasing this concentration led to a higher fermentable reducing sugar content.
Fig. 4A shows the reducing sugar released after enzymatic hydrolysis by Termamyl 120L different concentrations.
The results indicated that the higher the enzyme concentration was, the higher the fermentable reducing sugar content.
The use of Termamyl 120L(45 U) released 14.16 g L−1 reducing sugars after a 60 min of incubation.
Izmirlioglu and Demirci (2012) indicated that the liquefaction stage alone was insufficient and a supplementary saccharification stage was required.
For this reason, the use of enzyme combination (Termamyl 120L and amyloglucosidase) was necessary for an effective PPW hydrolysis.
Therefore, reducing sugars release was determined using 45 U of Termamyl 120L combined to different concentrations of amyloglucosidase concentrations (Fig. 4B).
The enzyme combination with the highest release of reducing sugars (51 g L−1) was Termamyl 120L 45 U + amyloglucosidase 9 U.
At higher enzymes doses, no significant increase in reducing sugars release was obtained.
Therefore, this combination was retained to optimize the best PPW concentration for optimal release of reducing sugars.
Different concentrations (ranging from 1.25% to 17.5%) were tested (Fig. 4C).
The results showed that at low PPW concentration, very low amounts of reducing sugars were released but increasing this concentration led to a higher fermentable reducing sugar content.
การแปล กรุณารอสักครู่..
