It should be remembered, however, that these tests are used when there are no apparent stains, meaning that very little blood is likely to be recovered, and thus any degradation can be critical for subsequent genotyping. This hypothesis should be pursued by further studies. Also, a comparison with other DNA extraction methods should be performed. The organic method for DNA extraction is the chosen method for scarce and degraded samples in our lab and the forensic community, however a future study should be done with other extraction protocols. Also, in a future study, evaluation of STRs profiles should be performed as a confirmation of Real Time data. DNA genotyping was not performed in this study because there was only quantitative evaluation, but this confirmation is one of the goals of our next studies.