Highlights
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Fecal samples were positive for Salmonella Kentucky but none yielded Enteritidis.
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Hens given both serotypes orally or by contact had fewer positive samples than those given Enteritidis.
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Giving both serotypes compared to only Salmonella Enteritidis altered egg production.
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Environmental Salmonella may impact the recovery of more pathogenic serotypes.
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ISR serotyping is useful for evaluating multiple Salmonella serotypes on-farm.