Fig. 3. Effect of barley growth in epibrassinolide (epiBL) on the transcriptional activity in Fusarium-infected stem tissue compared with growth in soil amended
with water. Plants were grown in soil amended with either water or 20 μM epiBL. After 10 days, stem bases were treated with Fusarium culmorum conidia in
Tween 20 and 1% agar. Microarray analysis was conducted using tissue harvested A, 24 and B, 48 h post pathogen inoculation. RNA was extracted from stem
base tissue and results are based on independent microarray analysis for three replica experiments and represent transcripts significantly affected by epiBL
treatment (≥2 and ≤–2-fold change in expression; P ≤ 0.05). Genes were assigned to functional categories using the Munich Information Center for Protein
Sequence Functional Catalogue scheme (release 2.1) (http://mips.helmholtz-muenchen.de/proj/funcatDB/search_main_frame.html).