Using controlled, laboratory fermentations and the bacterial inhibitors
nisin and lysozyme, we evaluated the contribution of LAB to
cocoa bean fermentations. Based on microbiological and chemical
criteria, discussed in the following sections, these small scale (5 kg)
fermentations followed the behavior of larger, commercial scale cocoa
bean fermentations. Pereira et al. (2012) also noted similar conclusions
when comparing small and large scale cocoa fermentations. These
inhibitors did not change the normal contribution of yeasts and acetic
acid bacteria to the fermentations. In one trial (2011 beans),
they completely inhibited the growth of LAB while in another trial
(2012 beans), they eliminated the contributions of L. pentosus and
L. fermentum and restricted the growth of L. plantarum by 100 fold
(b106 CFU/g) and to a shorter time frame. Thus, by comparing between
fermentations in the absence and presence of these inhibitors, we are
able to gain some insight into the contribution of LAB to the process
and cocoa bean quality
Using controlled, laboratory fermentations and the bacterial inhibitorsnisin and lysozyme, we evaluated the contribution of LAB tococoa bean fermentations. Based on microbiological and chemicalcriteria, discussed in the following sections, these small scale (5 kg)fermentations followed the behavior of larger, commercial scale cocoabean fermentations. Pereira et al. (2012) also noted similar conclusionswhen comparing small and large scale cocoa fermentations. Theseinhibitors did not change the normal contribution of yeasts and aceticacid bacteria to the fermentations. In one trial (2011 beans),they completely inhibited the growth of LAB while in another trial(2012 beans), they eliminated the contributions of L. pentosus andL. fermentum and restricted the growth of L. plantarum by 100 fold(b106 CFU/g) and to a shorter time frame. Thus, by comparing betweenfermentations in the absence and presence of these inhibitors, we areable to gain some insight into the contribution of LAB to the processand cocoa bean quality
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