Three days after treatment, sprout samples were collected
gently from the surface of the filter papers, frozen in liquid
nitrogen immediately and kept in polyethylene bags at
−70 ◦C for
the analysis of glucosinolates, total phenolic content, ascorbic acid,
anthocyanin and the ferric reducing ability of plasma (FRAP) value,
as well as myrosinase and PAL activity. For each treatment, three
replicates were analyzed.