The microcapsules remained
in the calcium chloride solution for 30 min (hardening time) and then
they were washed in a sieve (pore size 0.125 mm) using sterile distilled
water, pH 4.0. After ionic gelation, the microcapsules were coated by
complex coacervation by immersion (30 min) in a 4% (w/v) solution
of whey protein concentrate previously heat treated (80 °C for
15 min) and adjusted to pH 4.0.