Quantification of Substrate: Liquid samples were analyzed by High Performance Liquid
Chromatography (Agilent LC1200 Series, USA) equipped with refractive index detector (RI) for
glucose and co-substrate concentrations. Each sample was filtered through a non-sterile 0.2 Ilm
nylon membrane filter to remove cells and injected to Aminex HPX-87H column, 300 x 7.8 mm
(Biorad, USA) in the 50°C housing in column oven and eluted by 5 mM H2S04 with a flow rate 0.60
ml.min-1 [19].