Fishes were washed thoroughly with chilled water to remove slime and dirt.
Head and viscera were removed manually. Eviscerated fishes were washed with chilled water and used for meat separation.Meat separation was done mechanically with the help of meat separator (Toyo Seikan Kaisha Ltd., Tokyo, Japan) having a pore diameter of 3 mm. The fresh mince was used for actomyosin preparation.