Similarly, we observed that IGF-1 did
not interfere in any end point, including the estradiol
concentrations, evaluated during the in vitro culture of
bovine secondary follicles (Araújo et al., unpublished
observations). Therefore, the action of IGF-1 in bovine
oocyte and follicular development is strictly regulated
by the developmental stage, period of culture, and concentration
of IGF-1 used [114].