Using this MPCR technique, we analyzed 6 well-characterized reference strains and 46 clinical isolates, previously identified by the Vitek Automated system, from patients of the main hospitals of the Canary Islands.
The results are shown in Fig. 1. All enterococcal isolates gave rise to a 112 bp PCR fragment corresponding to part of the tuf gene, which is amplified by the Ent pair of primers.