Previous studies mainly focused on mean performance of all engine cylinders, while the imbalance that methanol entered in to each cylinder will pull down the whole engine performance such as decrease of engine power and torque, increase of exhaust emission. This work first proposed the definition of UED of DMDF engine calibrated by peak pressure and paid more attention to pressure comparison of each cylinder. In order to investigate the effect on UED cylinder-to-cylinder, the inlet duct injection of methanol is studied on a 4-cylinder turbocharged direct injection diesel engine and the cylinder pressure, COV , CA05, CA50 and CA90 of 4 cylinders are analyzed under different engine speeds and loads with the change of intake air temperature and MSP, the engine test combination is given in Table 1.The effect of above parameters on the UED of DMDF engine are analyzed to ensure high MSP used in diesel engine with each cylinders work more evenly.