a b s t r a c t
During several forays for ligninolytic fungi in different Spanish native forests, 35 white-rot basidiomycetes
growing on dead wood (16 species from 12 genera) and leaf litter (19 species from 10 genera) were
selected for their ability to decolorize two recalcitrant aromatic dyes (Reactive Blue 38 and Reactive Black
5) added to malt extract agar medium. In this study, two dye decolorization patterns were observed and
correlated with two ecophysiological groups (wood and humus white-rot basidiomycetes) and three
taxonomical groups (orders Polyporales, Hymenochaetales and Agaricales). Depending on the above
groups, different decolorization zones were observed on the dye-containing plates, being restricted to
the colony area or extending to the surrounding medium, which suggested two different decay strategies.
These two strategies were related to the ability to secrete peroxidases and laccases inside (white-rot
wood Polyporales, Hymenochaetales and Agaricales) and outside (white-rot humus Agaricales) of the
fungal colony, as revealed by enzymatic tests performed directly on the agar plates. Similar oxidoreductases
production patterns were observed when fungi were grown in the absence of dyes, although the set
of enzyme released was different. All these results suggest that the decolorization patterns observed
could be related with the existence of two decay strategies developed by white-rot basidiomycetes
adapted to wood and leaf litter decay in the field.
a b s t r a c tDuring several forays for ligninolytic fungi in different Spanish native forests, 35 white-rot basidiomycetesgrowing on dead wood (16 species from 12 genera) and leaf litter (19 species from 10 genera) wereselected for their ability to decolorize two recalcitrant aromatic dyes (Reactive Blue 38 and Reactive Black5) added to malt extract agar medium. In this study, two dye decolorization patterns were observed andcorrelated with two ecophysiological groups (wood and humus white-rot basidiomycetes) and threetaxonomical groups (orders Polyporales, Hymenochaetales and Agaricales). Depending on the abovegroups, different decolorization zones were observed on the dye-containing plates, being restricted tothe colony area or extending to the surrounding medium, which suggested two different decay strategies.These two strategies were related to the ability to secrete peroxidases and laccases inside (white-rotwood Polyporales, Hymenochaetales and Agaricales) and outside (white-rot humus Agaricales) of thefungal colony, as revealed by enzymatic tests performed directly on the agar plates. Similar oxidoreductasesproduction patterns were observed when fungi were grown in the absence of dyes, although the setof enzyme released was different. All these results suggest that the decolorization patterns observedcould be related with the existence of two decay strategies developed by white-rot basidiomycetesadapted to wood and leaf litter decay in the field.
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