Two air-pulsed fluidized bed glass bioreactors (1.5 L) were set up in parallel to treat veterinary hospital wastewater: one inoculated with T. Versicolor in form of pellets and the other one, non- inoculated, was used as a control with no biomass except the wastewater-associated bacterial communities. A sterile reactor could not be set up to measure the abiotic degradation due to the inability to sterilize the water (heat, enzyme treatment, filtration, etc.) without affecting the stability of antibiotics. Pellets of T. versicolor were added at 2.0 g dry cell weight (DCW) L 1. Tem- perature was set up at 25 C and pH was controlled to be constant at 4.5 ± 0.5 by HCl 1 M or NaOH1 M addition. Bioreactors were operated in fed-batch mode for nutrients: glucose and ammonia tartrate were added at 277 mg g dry cell weight (DCW) 1 d 1 and 0.619 mg g DCW 1 d 1 respectively in pulses of 0.6 min h 1 from a concentrated stock. Addition rate was adjusted to avoid glucose accumulation in the media. Glucose concentration and laccase ac- tivity were monitored to assure the good performance of the bioreactor, as previously described. Liquid samples of approxi- mately 50 mL were taken at the beginning and at the end of the experiment (after 15 days) by triplicate for the analytical pro- cedures. The samples were kept in the dark to avoid the photo- degradation of some of the antibiotics.