The peptides of interest were examined by gel electrophoresis.
Gel electrophoresis. Tricine–SDS–PAGE [24] and acid–urea–PAGE [25] were performed in minigels using a SE 250 vertical gel unit (Hoefer, San Francisco, CA). Gels were stained with a solution that contained 1 g of Coomassie brilliant blue R-250 (Sigma), 270 ml methanol, 630 ml water, and 150 ml formaldehyde, and then were destained in 5% acetic acid.
The peptides of interest were examined by gel electrophoresis.Gel electrophoresis. Tricine–SDS–PAGE [24] and acid–urea–PAGE [25] were performed in minigels using a SE 250 vertical gel unit (Hoefer, San Francisco, CA). Gels were stained with a solution that contained 1 g of Coomassie brilliant blue R-250 (Sigma), 270 ml methanol, 630 ml water, and 150 ml formaldehyde, and then were destained in 5% acetic acid.
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