The dried
extract of C. asiatica (100 mg) was vigorously
shaken with 10 ml of acetone - hexane (4:6) for 1
min and filtered through filter paper (Whatman
No. 4). The absorbance of the filtrate was
measured at 453, 505, 645 and 663 nm
spectrophotometrically. ß-carotene content was
calculated according to the following equations