2.2. Bio-processing of groundnut oil cake through solid statefermentationInoculum was prepared in 250 ml conical flasks containing 45 mlselective tannic acid broth inoculated with 5 ml of a 24 h cul-ture. The flasks were then incubated at 35◦C for 48 h and theinoculum thus prepared was used for the inoculation of the solidsubstrate medium. Total viable cell count of the yeast strain in the48 h broth culture was determined by Colony Count Technique(Mandal and Ghosh, 2013b) and the cell density was found as7.5 × 107cells ml−1.GOC (de-oiled) was purchased from local market, dried at 80◦Cin hot air oven, grounded, passed through a fine mesh sieve (400 min diameter) to ensure homogeneity and the powder was usedfor fermentation. The powdered GOC was moistened with 60%w/v liquid basal medium containing (g l−1) K2HPO4(0.5); KH2PO4(0.5); MgSO4(0.5); NH4Cl (1), CaCl2(0.01) having a pH of 6.5 andautoclaved at 121◦C and 15 lbs for 20 min for sterilization. The ster-ilized oil cake was fermented with P. kudriavzevii at the rate of3 × 107yeast cells g−1of dried oil cake for 15 days at 34◦C in anincubator