9.Stimuli-ResponsivePolymers
Recently it has been shown that smart polymers can act as a pseudochaperonins and precipitation of the protein from its denatured solution by such polymers can yield refolded active protein [69, 70]. Such polymers exist in soluble state in aqueous buffers and can be made insoluble by application of a stimulus. The latter can be a pH change, temperature change, or a change in the concentration of chemical species. This process is reversible. Table 2 represents the smart polymers which are used in refolding of recombinant proteins.