Briefly, 200 mL of sterile distilled water was distributed in the outer wells of the microplate (Falcon 3072, Becton Dickinson, Lincoln Park, NJ, USA); extracts and pure compound were diluted in dimethyl sulfoxide (DMSO)
(Amresco), and serial twofold dilutions from 250 to 1.9 mg/mL were made in Middlebrook 7H9 medium supplemented with
OADC. Isoniazid was used as the reference drug at concentrations from 1.0 to 0.007 mg/mL.
Briefly, 200 mL of sterile distilled water was distributed in the outer wells of the microplate (Falcon 3072, Becton Dickinson, Lincoln Park, NJ, USA); extracts and pure compound were diluted in dimethyl sulfoxide (DMSO)(Amresco), and serial twofold dilutions from 250 to 1.9 mg/mL were made in Middlebrook 7H9 medium supplemented withOADC. Isoniazid was used as the reference drug at concentrations from 1.0 to 0.007 mg/mL.
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