The C: N ratio was maintained at 10: 1 using ammonium sulphate as nitrogen and wheat flour as carbon source. Inorganic nitrogen source was applied up to the 3rd day (72 h) while addition of carbohydrate was continued till the 4th day. Thiswas done for maximumutilization of left over inorganic nitrogen and to reduce the chance of occurrence of inorganic nitrogen in the form of total ammonia nitrogen in the collected biofloc.