After 24 hours of aeration, 1 liter of supernatant
water was replaced by the synthetic wastewater using
fill and draw method. Samples were taken to determine
ammonia nitrogen and MLSS in the reactor at 5,
35, 65, 95 and 120 minutes, respectively. At the
same time, ten cubes were taken to examine
the change in biomass concentration inside the biocarrier.