The current work aims to shorten the juvenility phase and to
induce normal flowering of a temperate orchid herb D. nobile in
vitro. We have established a high frequency in vitro flowering
protocol, using seeds from self-pollinated seed pods as starting
materials. Growing seedling in medium with PP333 + TDZ followed
by culture in lower temperature on flower development was also
investigated. These findings are important for molecular and
genetic studies on the mechanisms of flower induction and for
advancing orchid breeding programs.