The second and third instars of C. villeneuvi were
obtained from two unknown human male remains that were
transferred for investigation to the Department of Forensic Medicine, Chiang Mai University, Thailand, on 4 February
2004. These remains were discovered in the forested area of
Muang Pan District, Lampang province, northern Thailand.
During examination, numerous fly larvae (maggots) were
found under their heads and bodies. Some larval specimens
were collected and killed by transferring them into a beaker
containing near boiling water [3] for a few minutes. The
dead larvae were cut using a sharp blade at two sites to obtain
three body portions (Fig. 1). Each part was mounted by
transferring them onto a clean glass slide after a few drops of
Entellan1 (Merck: Germany) had been placed on it. A cover
slip was placed over the specimens and an examination for
morphological characters was made under a light microscope
(Olympus1, Japan) equipped with a calibrated eye
piece micrometer. The images were recorded with a digital
camera (Olympus Camedia, C-4040Zoom1, Japan). Moreover,
some larval specimens were reared to confirm the
species of C. villeneuvi in the laboratoryat the Department
of Parasitology, Faculty of Medicine, Chiang Mai University,
using pork liver as food source.