2.3.8. Leaching experiment of 2,4-D and photoresponsive
polymers based on coumarin-2,4-D conjugate (3a–b). The
leaching of pesticide (2,4-D) and photoresponsive polymers (3a–
b) through a thin layer of soil was carried out in a Buchner
funnel with a diameter of 8 cm.39 A thin layer of soil (loam soil)
weighing 50 g was deposited on a Whatman lter paper in
a funnel. The tested compound weighing 1 mg (M0) was applied
to the top of the soil in the funnel and then the soil was covered
with another piece of Whatman lter paper. The funnels were
irrigated with 40 mL of water at 2 h intervals. Each sample was
irrigated for a total of eight times. Aer each irrigation, the
leachate was collected and analyzed on uorescence spectrometry. The amount of compound leached each time (Mt) was
calculated with respect to the initial concentration of the
compound (M0). For each sample, the leaching test was performed in triplicate