Zein Wlms were produced as described in Padgett
et al. (1998). BrieXy, 1.4 g corn zein was dissolved with
8.1 mL of ethanol (97%) by mixing slowly with a magnetic
stirrer for 25 min. 0.39 mL glycerol was then
added to the medium and the temperature of the mixture
was increased until it started to boil. The mixing
was then ceased and the Wlm solution was boiled for
5 min. After cooling to room temperature, diVerent
amounts of lysozyme and/or disodium EDTA were
added to the mixture to achieve 187–1524 U/cm2
(63–
530 g/cm2
) and 180 g/cm2 Wnal concentrations in
dried Wlms, respectively. The mixture was further
stirred for 25 min and 4.3 g of it was spread evenly onto
a 8.5 £ 8.5 cm glass plate, previously cleaned with ethanol.
The plates were placed in an incubator (Memmert,
108 L, Model BE 500, Germany) and the Wlms were
dried at room temperature for 24 h by keeping the incubator
door half open. After drying, the Wlms were
peeled from the glass plates carefully and 6 £ 6 cm
pieces cut from the middle of Wlms were used in all
tests. The average thicknesses of the Wlms obtained by
this procedure changed between 0.139 and 0.142 mm
for control, lysozyme incorporated or lysozyme and
disodium EDTA incorporated zein Wlms (minimum 20
measurements were conducted at diVerent points of
each kind of Wlm with a micrometer)
Zein Wlms were produced as described in Padgettet al. (1998). BrieXy, 1.4 g corn zein was dissolved with8.1 mL of ethanol (97%) by mixing slowly with a magneticstirrer for 25 min. 0.39 mL glycerol was thenadded to the medium and the temperature of the mixturewas increased until it started to boil. The mixingwas then ceased and the Wlm solution was boiled for5 min. After cooling to room temperature, diVerentamounts of lysozyme and/or disodium EDTA wereadded to the mixture to achieve 187–1524 U/cm2 (63–530 g/cm2) and 180 g/cm2 Wnal concentrations indried Wlms, respectively. The mixture was furtherstirred for 25 min and 4.3 g of it was spread evenly ontoa 8.5 £ 8.5 cm glass plate, previously cleaned with ethanol.The plates were placed in an incubator (Memmert,108 L, Model BE 500, Germany) and the Wlms weredried at room temperature for 24 h by keeping the incubatordoor half open. After drying, the Wlms werepeeled from the glass plates carefully and 6 £ 6 cmpieces cut from the middle of Wlms were used in alltests. The average thicknesses of the Wlms obtained bythis procedure changed between 0.139 and 0.142 mmfor control, lysozyme incorporated or lysozyme anddisodium EDTA incorporated zein Wlms (minimum 20measurements were conducted at diVerent points ofeach kind of Wlm with a micrometer)
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