Eight in vitro (on root organs) and four in vivo (on plants) produced AMF single species cultures (i.e., cultures established via either a single spore or multiple spores or a single root piece containing several spores/vesicles from the
same species) belonging to different genera were considered (Table 1). All the single species cultures were provided by GINCO (http://www.mycorrhiza.be/ginco-bel/index.php). An in vitro culture of Rhizophagussp. MUCL 43204, which was previously cryopreserved at−130 °C using the encapsulationdrying protocol (Lalaymia et al.2012), was used as control to evaluate the success of the cryopreservation protocol.