24 Components were identified in the fruits, leaves and stems EOs of A. guilinensis. All three EOs had broad-spectrum and strong antibacterial activities against four tested foodborne bacteria with the DIZ of leaves oil (16.8 mm) close to streptomycin (18.3 mm). Our study showed considerable difference in antibacterial mechanism between A. guilinensis EOs and streptomycin. The increasing permeability of bacterial membrane and direct damage to cell structure may be an important mode of action of A. guilinensis EOs. In addition, the hemolytic rates of three EOs on human RBCs were below 1%. Therefore, A. guilinensis EOs have potential applications as natural and safe food preservatives.