Evaluation of the newly developed method on the isolates from the food processing plant showed an exceptionally high identifi- cation rate of 92.6%. As described previously, L. seeligeri was problematic in that it exhibited 2 peak patterns in HRMA of rarA; however, for identification of food industry isolates the present method had a high success rate of 84.6% for L. seeligeri, and all strains identified as L. seeligeri by 16S rDNA sequencing were also identified as such by HRMA. From these results, it is likely a non- issue for actual daily testing in factory. The typestrain of L. seeligeli was thought to have untypical rarA sequence, because the 22 strains of L. seeligeri isolated from food industry showed same HRM profile as the isolated strain L. seeligeri 2±1. Additionally, differentiation between the 2 strains by Tm value analysis of ldh on isolates showed that out of 33 strains, 32 were correctly identified, demonstrating the practicality of the criteria in the present study.