ฉันรักแปลCT was extracted from L. corniculatus leaves by the method of Terrill et al. (1992)
with the following modifications: L. corniculatus leaves were homogenized in 70% acetone
containing 0.1% ascorbic acid, and plant pigments were removed by three rounds of methylene
chloride extraction. The remaining CT fraction was freeze-dried, redissolved in 50%
methanol (v/v) and purified by gel filtration using a Sephadex LH-20 column (Pharmacia,
Uppsala, Sweden) washing with 1000 ml of 50% methanol (v/v) and eluting with 200 ml