MATERIALS AND METHODS
Pure seeds of three roselle cultivars: Aswan, Sewa and Sudan-1 were obtained from the production program of the Arid Land Agriculture Department, in the Agricultural Research Station, King Abdulaziz University, Hada Al-Sham, Saudi Arabia. A randomized complete block design with four replications was used. Seed samples were separately milled, and oil was extracted using Soxtech with N-hexane according to AOAC (2000). Defatted seeds were used to determine ash and fiber contents in seeds (AOAC, 2000).
Protein andamino acids composition
Roselle seed protein contents were determined using Kjetech